pulsed-field gel electrophoresis

Abbreviation: PFGE
A procedure used to separate very large (50 kbp to several Mbp) DNA molecules by alteRNAting the direction of electric current in a pulsed manner across a gel.

Glossary of Biotechnology for Food and Agriculture . 2015.

Look at other dictionaries:

  • pulsed-field gel electrophoresis — pəls(t) .fē(ə)ld n gel electrophoresis that is used esp. to separate large fragments of DNA and that involves changing the direction of the electric current periodically in order to minimize overlap of the spots due to diffusion called also pulse …   Medical dictionary

  • Pulsed field gel electrophoresis — Historical Background Standard gel electrophoresis techniques for separation of DNA molecules provided huge advantages for molecular biology research. However, many limitations existed with the standard protocol in that it was unable to separate… …   Wikipedia

  • pulsed-field gel electrophoresis — ˌ ̷ ̷ ˌ ̷ ̷ noun : gel electrophoresis that is used especially to separate large fragments of DNA and that involves changing the direction of the electric current periodically in order to minimize overlap of the spots due to diffusion …   Useful english dictionary

  • Gel electrophoresis — apparatus – An agarose gel is placed in this buffer filled box and electrical field is applied via the power supply to the rear. The negative terminal is at the far end (black wire), so DNA migrates toward the camera. Classification… …   Wikipedia

  • Gel electrophoresis of nucleic acids — Digital printout of an agarose gel electrophoresis of cat insert plasmid DNA …   Wikipedia

  • pulsed field gradient gel electrophoresis — pulsed field gradient gel electrophoresis. См. гель электрофорез в градиенте пульсирующего поля. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • pulsed-field electrophoresis — a method of gel electrophoresis used to separate fragments of DNA as long as several million bases by subjecting the gel to an electrical current alternately delivered from two angles in timed intervals, which minimizes diffusion of large… …   Medical dictionary

  • Agarose gel electrophoresis — is a method used in biochemistry and molecular biology to separate DNA, or RNA molecules by size. This is achieved by moving negatively charged nucleic acid molecules through an agarose matrix with an electric field (electrophoresis). Shorter… …   Wikipedia

  • Electrophoresis — is the most well known electrokinetic phenomenon. It was discovered by Reuss in 1809. [Reuss, F.F. Mem.Soc.Imperiale Naturalistes de Moscow , 2, 327 1809] He observed that clay particles dispersed in water migrate under influence of an applied… …   Wikipedia

  • electrophoresis — A ubiquitous molecular biology technique, with many variants, used to resolve complex mixtures of macromolecules into their components. Its principle is to subject samples to an electric field applied across a porous matrix. Molecules will… …   Glossary of Biotechnology

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”

We are using cookies for the best presentation of our site. Continuing to use this site, you agree with this.