cDNA cloning
A method of cloning the coding sequence of a gene, starting with its mRNA transcript.

Glossary of Biotechnology for Food and Agriculture . 2015.

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  • CDNA library — A cDNA library is a collection of cloned cDNA (complementary DNA) fragments. cDNA is produced from fully transcribed mRNA found in the nucleus and therefore contains only the coding regions of an organism. While information in cDNA libraries is a …   Wikipedia

  • Cloning vector — The pGEX 3x plasmid is a popular cloning vector. A cloning vector is a small piece of DNA into which a foreign DNA fragment can be inserted. The insertion of the fragment into the cloning vector is carried out by treating the vehicle and the… …   Wikipedia

  • Molecular cloning — refers to a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms.[1] The use of the word cloning refers to the fact that the method involves the …   Wikipedia

  • GFP-cDNA — The GFP cDNA project documents the localisation of proteins to subcellular compartments of the eukaryotic cell applying fluorescence microscopy. Experimental data are complemented with bioinformatic analyses and published online in a database. A… …   Wikipedia

  • Rapid Amplification of cDNA Ends — RACE, or Rapid Amplification of cDNA Ends, is a technique used in molecular biology to obtain the partial sequence of an RNA transcript found within a cell. RACE results in the production of a DNA copy of the RNA sequence of interest, produced… …   Wikipedia

  • IMAGE cDNA clones — are a collection of DNA vectors containing cDNAs from various organisms including human, mouse, rat, non human primates, zebrafish, pufferfish, Xenopus (frogs), and cow. [http://image.llnl.gov/image/html/iresources.shtml] Together they represent… …   Wikipedia

  • functional cloning — (= expression cloning) Strategy for cloning a desired gene that is based on some property (antigenicity, ligand binding, etc.) of the expressed gene. For example, a cDNA library could be produced in a eukaryotic expression vector, and transfected …   Dictionary of molecular biology

  • expression cloning — Method of gene cloning based on transfection of a large number of cells with cDNAs in an expression vector (eg. a cDNA library), then screening for a functional property (eg. binding of a radiolabelled hormone to identify receptors, or induction… …   Dictionary of molecular biology

  • PSG1 (gene) — Pregnancy specific beta 1 glycoprotein 1 (PSG1) also known as CD66f (Cluster of Differentiation 66f), is a human gene.cite web | title = Entrez Gene: PSG1 pregnancy specific beta 1 glycoprotein 1| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db …   Wikipedia

  • CES1 — Carboxylesterase 1 (monocyte/macrophage serine esterase 1), also known as CES1, is a human gene.cite web | title = Entrez Gene: CES1 carboxylesterase 1 (monocyte/macrophage serine esterase 1)| url =… …   Wikipedia

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