affinity tag
An amino acid sequence that has been engineered into a protein to make its purification easier. The tag could be another protein or a short amino acid sequence, allowing purification by affinity chromatography.

Glossary of Biotechnology for Food and Agriculture . 2015.

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  • Affinity chromatography — is a method of separating biochemical mixtures and based on a highly specific interaction such as that between antigen and antibody, enzyme and substrate, or receptor and ligand. Contents 1 Uses 2 Principle 3 Batch and column setup …   Wikipedia

  • purification tag — See: affinity tag …   Glossary of Biotechnology

  • Polyhistidine-tag — A polyhistidine tag is an amino acid motif in proteins that consists of at least six histidine ( His ) residues, often at the N or C terminus of the protein. It is also known as hexa histidine tag, 6xHis tag, and by the trademarked name His tag… …   Wikipedia

  • Tandem Affinity Purification — The Tandem Affinity Purification (TAP) is a technique for studying protein protein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end. The protein of interest with the TAP tag first binds to beads… …   Wikipedia

  • Protein tag — Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes …   Wikipedia

  • FLAG-tag — FLAG tag, or FLAG octapeptide, is a polypeptide protein tag that is added to a recombinant expressed protein. It is made by inserting a vector into the DNA sequence coding for that specific protein. It can be used for affinity chromatography,… …   Wikipedia

  • Processor affinity — is a modification of the native central queue scheduling algorithm.Each task (be it process or thread) in the queue has a tag indicating its preferred / kin processor.At allocation time, each task is allocated to its kin processor in preference… …   Wikipedia

  • Protein-Tag — Als Protein Tag (engl. tag = Markierung) werden in der Biochemie verschiedene meist kurze Aminosäuresequenzen bezeichnet, mit deren Hilfe Proteine markiert werden können. Für verschiedene Zwecke sind verschiedenen Markierungen unterschiedlich gut …   Deutsch Wikipedia

  • Myc-tag — A myc tag is a polypeptide protein tag derived from the c myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from… …   Wikipedia

  • his tag — (= histidine tag) Epitope tag based on a short stretch (ca 6) of histidine residues. In addition to detection with anti polyhistidine antibodies, such tags permit easy protein purification on nickel based affinity columns …   Dictionary of molecular biology

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