Gram staining

A technique to distinguish between two major bacterial groups, based on whether or not their cell wall retains the Gram stain. Gram-positive bacteria are stained dark purple, while Gram-negative bacteria are only faintly coloured. Stain retention is determined by the structure of the cell wall.

Glossary of Biotechnology for Food and Agriculture . 2015.

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  • Gram staining — (or Gram s method) is an empirical method of differentiating bacterial species into two large groups (Gram positive and Gram negative) based on the chemical and physical properties of their cell walls.cite book | last = Bergey | first = David H.… …   Wikipedia

  • Gram staining — noun Grams method of distinguishing the two main groups of bacteria …   Wiktionary

  • Staining — is an auxiliary technique used in microscopy to enhance contrast in the microscopic image.In biochemistry it involves adding a class specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a… …   Wikipedia

  • Gram-positive bacteria — Gram positive bacteria, stained purple, of both the bacillus (“rod shaped”) and coccus (spherical) forms.  A few Gram negative bacteria are also present, stained pink.  Numbered ticks are eleven (11) microns apart. Gram positive… …   Wikipedia

  • Gram-negative — bacteria lose the crystal violet stain (and take the color of the red counterstain) in Gram s method of staining. This is characteristic of bacteria that have a cell wall composed of a thin layer of a particular substance (called peptidoglycan).… …   Medical dictionary

  • Gram-positive — bacteria retain the color of the crystal violet stain in the Gram stain. This is characteristic of bacteria that have a cell wall composed of a thick layer of a particular substance (called peptidologlycan). The Gram positive bacteria include… …   Medical dictionary

  • Gram (disambiguation) — Gram is a unit of measurement of mass. Otherwise, gram may refer to: gram, the Greek based suffix meaning drawing or representation. Contents 1 Places 2 People …   Wikipedia

  • Gram-negative bacteria — Microscopic image of Gram negative Pseudomonas aeruginosa bacteria (pink red rods). Gram negative bacteria are bacteria that do not retain crystal violet dye in the Gram staining protocol.[1] In a Gram …   Wikipedia

  • Gram stain — The Danish bacteriologist J.M.C. Gram (1853 1938) devised a method of staining bacteria using a dye called crystal (gentian) violet. Gram s method helps distinguish between different types of bacteria. The gram staining characteristics of… …   Medical dictionary

  • Gram's method — noun a staining technique, using a sequence of crystal violet, iodine and safranine, used to differentiate bacteria into two large groups of species Syn: Gram staining …   Wiktionary

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