polylinker

A synthetic segment of DNA, designed to include a number of different restriction endonuclease sites. When ligated to a DNA fragment that is to be cloned, this enables a wide choice of restriction endonucleases to be used for the cloning process.
Synonym: multiple cloning site (MCS).

Glossary of Biotechnology for Food and Agriculture . 2015.

Look at other dictionaries:

  • polylinker — polylinker. См. полилинкер. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • Polylinker — Ein Polylinker oder auch Multiple Cloning Site (MCS) ist ein künstlich geschaffenes DNA Oligonukleotid aus rund 50bp, welches in ein Plasmid eingesetzt wird und dessen Sequenz direkt hintereinander verschiedene Restriktionsschnittstellen für… …   Deutsch Wikipedia

  • polylinker — noun A short sequence of DNA containing several restriction enzyme recognition sites that is contained in cloning vectors …   Wiktionary

  • polylinker — An inserted sequence of DNA in recombinant DNA vectors consisting of a cluster of numerous restriction endonuclease sites unique in the plasmid; also called restriction site bank and polycloning site …   Medical dictionary

  • полилинкер — polylinker полилинкер. Искусственная нуклеотидная последовательность в составе векторной ДНК <vector>, содержащая несколько перекрывающихся сайтов узнавания рестриктазами <restriction endonucleases>. (Источник: «Англо русский толковый …   Молекулярная биология и генетика. Толковый словарь.

  • Multiple cloning site — A multiple cloning site (MCS), also called a polylinker, is a short segment of DNA which contains many (up to 20) restriction sites a standard feature of engineered plasmids.[1] Restriction sites within an MCS are typically unique, occurring only …   Wikipedia

  • PBluescript — In genetics, pBluescript (pBS) or pBluescript II is a commercially available phagemid containing several useful sequences for use in cloning with bacteriophage. The sequences include a polylinker sequence (MCS), antibiotic resistance sequence to… …   Wikipedia

  • Vector de clonación — Los vectores de clonación son moléculas transportadoras que transfieren y replican fragmentos de ADN que llevan insertados. Para que sirva de vector, una molécula debe ser capaz de replicarse junto con el fragmento de ADN que transporta. También… …   Wikipedia Español

  • pBluescript — In genetics, pBluescript (pBS) or pBluescript II is a commercially available phagemid containing several useful sequences for use in cloning with bacteriophage. The sequences include a polylinker sequence (MCS), antibiotic resistance sequence to… …   Wikipedia

  • Plasmid — Figure 1: Illustration of a bacterium with plasmid enclosed showing chromosomal DNA and plasmids. In microbiology and genetics, a plasmid is a DNA molecule that is separate from, and can replicate independently of, the chromosomal DNA …   Wikipedia

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