random mutagenesis

A non-directed change of one or more nucleotide pairs in a DNA molecule.

Glossary of Biotechnology for Food and Agriculture . 2015.

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  • Mutagenesis — (pronounced /mjuːtəˈdʒɛnɪsɪs/) is a process by which the genetic information of an organism is changed in a stable manner, resulting in a mutation. It may occur spontaneously in nature, or as a result of exposure to mutagens. It can also be… …   Wikipedia

  • Random chimeragenesis on transient templates — (RACHITT) is a method to perform molecular mutagenesis at a high recombination rate. DNA shuffling is a similar but less powerful method used in directed evolution experiments. References * Coco, W.M. et al. Nat. Biotechnol. 19, 354–359 (2001) …   Wikipedia

  • Insertional mutagenesis — is mutagenesis of DNA by the insertion of one or more bases.[1] Insertional mutations can occur naturally, mediated by virus or transposon, or can be artificially created for research purposes in the lab. Contents 1 Signature tagged mutagenesis 2 …   Wikipedia

  • Directed mutagenesis — Directed mutagenesis, also known as directed mutation, is a hypothesis proposing that organisms can respond to environmental stresses through directing mutations to certain genes or areas of the genome. The hypothesis was first proposed in 1988… …   Wikipedia

  • Signature tagged mutagenesis — Often abbreviated to STM, Signature Tagged Mutagenesis is a genetic technique used to study gene function. Recent advances in genome sequencing have allowed us to catalogue a large variety of organisms genomes, but the function of the genes they… …   Wikipedia

  • Knockout rats — Knockout rats=A knockout rat is a rat with a single gene disruption used for academic and pharmaceutical research. Knockout rats can mimic human diseases and are important tools for studying gene function (functional genomics) and for drug… …   Wikipedia

  • recombinant DNA technology — Genetics. any of various techniques for separating and recombining segments of DNA or genes, often employing a restriction enzyme to cut a gene from a donor organism and inserting it into a plasmid or viral DNA for transplantation into a host… …   Universalium

  • Genética inversa — La genética inversa es una disciplina genética que, partiendo del conocimiento de un fragmento de ADN clonado o secuenciado investiga sobre su función biológica alterando dicho ADN mediante mutación, generalmente a nivel masivo. Dicha mutación… …   Wikipedia Español

  • Ortsspezifische Mutagenese — Die Mutagenese (ein Kompositum, siehe auch Genesis bzw. Genese) ist die Erzeugung von Mutationen im Erbgut von Lebewesen. In der biologischen und medizinischen Forschung sowie in der Züchtung wird die Mutagenese eingesetzt, um erwünschte… …   Deutsch Wikipedia

  • Ligation-During-Amplification — (LDA) ist eine molekularbiologische Methode zur linearen Amplifikation von zirkulärer DNA (zum Beispiel Plasmid) mit nur einem sequenzspezifischen Primer unter Verwendung einer thermostabilen DNA Polymerase sowie einer thermostabilen DNA… …   Deutsch Wikipedia

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