single-strand conformational polymorphism

Abbreviation: SSCP
A technique for detection of mutations in a defined DNA sequence. Single-stranded polynucleotides are electrophoretically separated on non-denaturing gels. Intrachain base pairing results in a limited number of conformers stabilized by intrachain loops, and mutated DNA shows on electrophoresis an altered assortment of such conformers.

Glossary of Biotechnology for Food and Agriculture . 2015.

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