DNA sequencing

Procedures for determining the nucleotide sequence of a DNA fragment. Two common methods available:
1. The Maxam Gilbert technique, which uses chemicals to cleave DNA into fragments at specific bases; or, most commonly,
2. the Sanger technique (also called the di-deoxy or chain-terminating method) which uses DNA polymerase to make new DNA chains, in the presence of di-deoxynucleotides (chain terminators) to stop the chain randomly as it grows. In both cases, the DNA fragments are separated according to length by polyacrylamide gel electrophoresis, enabling the sequence to be read directly from the gel. The procedure has become increasingly automated and large-scale in recent years.

Glossary of Biotechnology for Food and Agriculture . 2015.

Look at other dictionaries:

  • DNA sequencing — Part of a series on Genetics Key components Chromosome DNA • RNA Genome Heredity Mutation Nucleotide Variation …   Wikipedia

  • DNA sequencing — DNR sekos nustatymas statusas T sritis augalininkystė apibrėžtis Metodas, kuriuo nustatomas azotinių bazių išsidėstymas (seka) DNR molekulėje. atitikmenys: angl. DNA sequencing rus. секвенирование ДНК ryšiai: sinonimas – DNR sekvenavimas …   Žemės ūkio augalų selekcijos ir sėklininkystės terminų žodynas

  • DNA sequencing — ▪ genetics  technique used to determine the nucleotide sequence of DNA (deoxyribonucleic acid). The nucleotide sequence is the most fundamental level of knowledge of a gene or genome. It is the blueprint that contains the instructions for… …   Universalium

  • DNA sequencing theory — is the broad body of work that attempts to lay analytical foundations for DNA sequencing. The practical aspects revolve around designing and optimizing sequencing projects (known as strategic genomics ), predicting project performance,… …   Wikipedia

  • chemical method of DNA sequencing — chemical method of DNA sequencing. = Maxam Gilbert method (см.). (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • chemical method of DNA sequencing — chemical method of DNA sequencing. См. химический метод секвенирования ДНК. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • Directed DNA sequencing — Successively sequencing DNA from adjacent stretches of a chromosome …   Medical dictionary

  • DNA nanoball sequencing — is a high throughput sequencing technology that is used to determine the entire genomic sequence of an organism. The method uses rolling circle replication to amplify small fragments of genomic DNA into DNA nanoballs. Fluorescent probes bind to… …   Wikipedia

  • DNA-encoded chemical library — DNA encoded chemical libraries (DEL) are a new technology for the synthesis and screening of collections of chemical compounds of unprecedented size and quality. DEL represents an advance in medicinal chemistry which bridges the fields of… …   Wikipedia

  • DNA-Sequenzierung — ist die Bestimmung der Nukleotid Abfolge in einem DNA Molekül. Die DNA Sequenzierung hat die biologischen Wissenschaften revolutioniert und die Ära der Genomik eingeleitet. Seit 1995 konnte durch DNA Sequenzierung das Genom von über 1000 (Stand:… …   Deutsch Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”

We are using cookies for the best presentation of our site. Continuing to use this site, you agree with this.